“Rapid detection of M. tuberculosis and Legionella from indoor air using modified impinger and poymerase chain reaction (PCR) methods.”

From this reason, Dr. Ramasoota tried to develop the rapid method that can detect 1-2 MTB or Legionella from indoor air. By combination of the in house developed impinger air collector with PCR to amplify bacterial DNA. It has become the world’s first report of developing realtime PCR that can detect only 1 bacilli of Legionella, and nested PCR that can detect only 2 bacilli of MDR-TB, from indoor air, within only 5-8 hours. Moreover, for the developed air collector system, by using domestic produced equipment, so the cost was 10 times less expensive than the imported impinger, but it has electric fan additionally build at the top, so higher amount of bio-aerosol can be collect.