Gnathostomiasis

Indirect ELISA (uses purified specific antigen)

5 ml of clot blood or serum

3

300 (Thai)

Dept. Microbiology and Immunology via OPD

Western blot analysis (uses crude extract of the infective larvae)

1-2 ml of serum

1

2,000 (Foreigner)

Western blot analysis (crude extract of infective larvae)

3, 1day *

200 (Thai)
1,000 (Foreigner)

Dept. Helminthology (Western blot only) via OPD, Department

Paragonimiasis

Indirect ELISA

5 ml of clot blood or serum

3

300 (Thai)

Dept. Microbiology and Immunology via OPD

Western blot analysis ( partially purified adult worm antigens are used for both assays )

1-2 ml of serum

1

2,000 (Foreigner)

Western blot analysis (crude extract of adult worms)

same

3, 1 day *

200 (Thai)
1,000 (Foreigner)

Dept. Helminthology via OPD

Trichinellosis

Indirect ELISA / dot - ELISA

5 ml of clot blood or serum

3/1

300 (Thai)

Dept. Microbiology and Immunology via OPD

Western blot analysis (the antigen used is either E-S, crude or affinity purified somatic antigens of the infective larvae)

1-2 ml of serum

1

2,000 (Foreigner)

Strongyloidosis

Indirect ELISA / dot - ELISA

5 ml of clot blood or serum

3/1

300 (Thai)

Dept. Microbiology and Immunology via OPD

Western blot analysis (uses partially purified antigens from filariform larvae)

1-2 ml of serum

1

2,000 (Foreigner)

Indirect ELISA (Molecular weight cut off antigen from filariform lavae) Western blot (crude extract of filariform larvae)

same

3, 1 day *

200 (Thai)
1,000 (Foreigner)

Dept. Helminthology via OPD

Toxocariasis

Indirect ELISA (Molecular wight cut off antigens from adult worms)

3

200 (Thai)

Dept. Helminthology via OPD

Western blot (crude extract of adult worms)

1,000 (Foreigner)

Angiostrongyliasis

Western blot (crude extract and partially purified antigens from adult worms)

same

3

200 (Thai)
1,000 (Foreigner)

Dept. Helminthology via OPD

Cysticercosis

Western blot (cystic fluid antigen)

same

3

200 (Thai)
1,000 (Foreigner)

Dept. Helminthology via OPD

Leptospirosis

Microagglutination method and dark - field microscopy using living leptospires

5 ml of clot blood 2)1-2 ml of serum 3) Blood absorbed onto filter paper

3

60

Dept. Microbiology and Immunology via OPD or directly to Leptospirosis unit, Dept. Microbiology and Immunology

Antigen detection assay

Urine sample (10 ml)

1

100

Directly at the Dept. Microbiology and Immunology

Cholera caused by serogroup O:1

Monoclonal antibody - based dot - blot ELISA using specific monoclonal antibodies to antigen A of V. cholerae serogroup O:1 (antigen detection); diagnostic kits are also available

Rectal swab in 1%alkaline peptone solution

1-2

150

Dept. Microbiology and Immunology via OPD

Watery stool

Food sample in 2% alkaline peptone solution

Water sample in 10% alkaline peptone solution

Cholera caused by serogroup O:139

Monoclonal antibody - based dot - blot ELISA using specific monoclonal antibodies (antigen detection)

Rectal swab in 1% alkaline peptone solution

1-3

150

Dept. Microbiology and Immunology via OPD

Watery stool