Immunodiagnoses of
various tropical infections
The following table summarizes the diseases/infections for
which diagnoses are provided; the serological tests used, the specimen(s)
required, the turnaround time for each test, the cost per test and the place
where specimens should be sent are included in the table.
| Disease/ Infection | Serological test used | Specimen required |
Time required (hours) |
Cost per test (Baht) |
Place where specimen should be sent |
| Gnathostomiasis |
1) Indirect ELISA (uses purified specific antigen) |
1) 5 ml of clot blood or serum |
3
|
300 (Thai) |
Dept. Microbiology and Immunology via OPD |
|
|
2) Western blot analysis (uses crude extract of the infective larvae) | 2) 1-2 ml of serum | 1 | 2,000 (Foreigner) | |
|
|
Western blot analysis (crude extract of infective larvae) | 3, 1 day* | 200 (Thai) 1,000 (Foreigner) |
Dept. Helminthology (Western blot only) via OPD, Department | |
| Paragonimiasis |
1) Indirect ELISA |
1) 5 ml of clot blood or serum | 3 | 300 (Thai) | Dept. Microbiology and Immunology via OPD |
| 2) Western blot analysis (partially purified adult worm antigens are used for both assays) | 2) 1-2 ml of serum | 1 | 2,000 (Foreigner) |
||
| 3) Western blot analysis (crude extract of adult worms) | 3) same | 3, 1 day* | 200 (Thai) 1,000 (Foreigner) |
Dept. Helminthology via OPD | |
| Trichinellosis | 1) Indirect ELISA/dot-ELISA | 1) 5 ml of clot blood or serum | 3/1 | 300 (Thai) | Dept. Microbiology and Immunology via OPD |
| 2) Western blot analysis (the antigen used is either E-S, crude or affinity purified somatic antigens of the infective larvae) | 2) 1-2 ml of serum | 1 | 2,000 (Foreigner) |
||
| Strongyloidosis | 1) Indirect ELISA/dot-ELISA | 1) 5 ml of clot blood or serum | 3/1 | 300 (Thai) | Dept. Microbiology and Immunology via OPD |
| 2) Western blot analysis (uses partially purified antigens from filariform larvae) | 2) 1-2 ml of serum | 1 | 2,000 (Foreigner) |
||
| 3) Indirect ELISA (Molecular weight cut off antigen from filariform lavae) Western blot (crude extract of filariform larvae) | 3) same | 3, 1 day* | 200 (Thai) 1,000 (Foreigner) |
Dept. Helminthology via OPD | |
| Toxocariasis | 1) Indirect ELISA (Molecular wight cut off antigens from adult worms) | same | 3 | 200 (Thai) | Dept. Helminthology via OPD |
| 2) Western blot (crude extract of adult worms) | 1,000 (Foreigner) |
||||
| Angiostrongyliasis | Western blot (crude extract and partially purified antigens from adult worms) | same | 3 | 200 (Thai) 1,000 (Foreigner) |
Dept. Helminthology via OPD |
| Cysticercosis | Western blot (cystic fluid antigen) | same | 3 | 200 (Thai) 1,000 (Foreigner) |
Dept. Helminthology via OPD |
| Leptospirosis | 1) Microagglutination method and dark-field microscopy using living leptospires | 1) 5 ml of clot blood 2) 1-2 ml of serum 3) Blood absorbed onto filter paper | 3 | 60 | Dept. Microbiology and Immunology via OPD or directly to Leptospirosis unit, Dept. Microbiology and Immunology |
| 2) Antigen detection assay | Urine sample (10 ml) | 1 | 100 | Directly at the Dept. Microbiology and Immunology | |
| Salmonella septicemia or Salmonellosis and Typhoid | Monoclonal antibody-based dot-blot ELISA using monoclonal antibodies specific to Salmonella core polysaccharide and antigen 9 (antigen detection) | 1) Three urine samples collected at 1 hour intervals (5 ml each) | 1-2 | 300 | Dept. Microbiology and Immunology via OPD |
| 2) Rectal swab, food/water samples in buffered peptone solution | |||||
| Cholera caused by serogroup O:1 | Monoclonal antibody-based dot-blot ELISA using specific monoclonal antibodies to antigen A of V. cholerae serogroup O:1 (antigen detection); diagnostic kits are also available | 1) Rectal swab in 1%alkaline peptone solution | 1-2 | 150 | Dept. Microbiology and Immunology via OPD |
| 2) Watery stool | |||||
| 3) Food sample in 2% alkaline peptone solution | |||||
| 4) Water sample in 10% alkaline peptone solution | |||||
| Cholera caused by serogroup O:139 | Monoclonal antibody-based dot-blot ELISA using specific monoclonal antibodies (antigen detection) | 1) Rectal swab in 1% alkaline peptone solution | 1-3 | 150 | Dept. Microbiology and Immunology via OPD |
| 2) Watery stool |
* Order should be made in advance