| 2001 |
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| Affinity purified oval antigen for diagnosis of Opisthorchiasis
viverrini. |
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| Wongsaroj, T., Sakolvaree, Y., Chaicumpa, W., Maleewong, W., Kitikoon, V., Tapchaisri, P.,
Chongsa-nguan, M., and Cross, J. H. |
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| Department of Microbiology and Immunology, Faculty of Tropical Medicine, Bangkok,
Thailand |
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| Abstract: Monoclonal antibodies (MAb) were raised against an oval antigen of the liver fluke
Opisthorchis viverrini which is the causative agent of a parasitosis, i.e. opisthorchiasis in Thailand. The antibodies were used in an affinity column to purify
the O. viverrini oval antigen from a crude extract of adult parasites by chromatography. The oval antigen was then used in a membrane (dot) ELISA for
detecting antibodies in serum samples of parasitologically confirmed Opisthorchis viverrini infected individuals (adult parasites were found in stools after
praziquantel treatment and salt purgation), as well as of individuals infected with other parasites and parasite-free controls. The MAb-based dot-ELISA
using the affinity purified O. viverrini oval antigen revealed 100% sensitivity, specificity and accuracy for detecting O. viverrini infection. The test is simple,
rapid and highly reproducible. Several samples can be tested at the same time without the requirement for special equipment or much increase in testing
time; thus it is suitable for mass screening for O. viverrini exposure, especially in new endemic areas. Furthermore using serum specimens could
increase patient and community compliance compared to the conventional parasitological survey which uses stool samples for the detection of O.
viverrini ova, without treatment and subsequent salt purgation, this conventional method shows a low sensitivity and is also unpleasant to both the sample
donors and the laboratory technicians which has historically shown a further negative impact on the final outcome |
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| Published in:Asian Pac.J.Allergy Immunol. 19[4], 245-258. 2001. |
