| 2008 |
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| Leptospira wolffii sp. nov., isolated from a human with suspected leptospirosis in
Thailand. |
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| Slack, A. T., Kalambaheti, T., Symonds, M. L., Dohnt, M. F., Galloway, R. L., Steigerwalt, A. G.,
Chaicumpa, W., Bunyaraksyotin, G., Craig, S., Harrower, B. J., and Smythe, L. D. |
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| WHO/FAO/OIE Collaborating Centre for Reference and Research on Leptospirosis, Western
Pacific Region, Communicable Diseases, Queensland Health Forensic and Scientific Services, Brisbane, Australia |
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| Abstract: A single Leptospira strain (designated Khorat-H2(T)) was isolated from the urine of an
adult male patient with suspected leptospirosis from the province of Nakornrachasima, Thailand. The isolate showed typical Leptospira motility and
morphology under dark-field microscopy. Cells were 10-13 mum long and 0.2 mum in diameter, with a wavelength of 0.5 mum and an amplitude of
approximately 0.3 mum. Phenotypically, strain Khorat-H2(T) did not grow at 13 degrees C but grew at 30 and 37 degrees C and in the presence of 8-
azaguanine. Serological identification using the microscopic agglutination test revealed that strain Khorat-H2(T) had no cross-reaction with any
recognized Leptospira serogroups. Phylogenetic analysis of the 16S rRNA gene sequence placed the novel strain within the radiation of the genus
Leptospira, with sequence similarities of 88.1-97.7 % to recognized Leptospira species. DNA-DNA hybridization against the type strains of the three most
closely related Leptospira species was used to confirm the results of the 16S rRNA sequence analysis. The G+C content of strain Khorat-H2(T) was 41.8
mol%. On the basis of phenotypic, serological and phylogenetic data, strain Khorat-H2(T) represents a novel species of the genus Leptospira, for which
the name Leptospira wolffii sp. nov. is proposed. The type strain is Khorat-H2(T) (=WHO LT1686(T) =KIT Khorat-H2(T)) |
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| Published in:Int.J.Syst.Evol.Microbiol. 58[Pt 10], 2305-2308. 2008. |
