| 2009 |
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| Development of single-tube mutiplex RT-PCR for dengue virus
typing. |
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| Maneekan, P., Jittmittraphap, A., Leaungwutiwong, P., Kaewkangwal, J., and Luplertlop, N.
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| Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok,
Thailand |
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| Abstract: This study evaluated a single-tube multiplex RT-PCR with a primer focusing on
nonstructural protein region 5 (NS5) and a highly conserved region for dengue virus serotyping. The method was compared with conventional PCR. This
new method had a sensitivity of 96.7% and a specificity of 96.7% for disease detection. The new method also proved suitable for use in the field as it
reduces time and decreases risk of contamination |
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| Published in:Southeast Asian J.Trop.Med.Public Health 40[6], 1254-1258. 2009. |
