 |
||||
|
||||
| 2007 |
Enhancement of Legionella pneumophila culture isolation from microenvironments by macrophage infectivity potentiator (mip) gene-specific nested polymerase chain reaction. |
Nintasen, R., Utrarachkij, F., Siripanichgon, K., Bhumiratana, A., Suzuki, Y., and Suthienkul, O. |
Department of Microbiology, Faculty of Public Health, Mahidol University, 420/1 Rajvithi Road, Rajthewee, Bangkok 10400, Thailand |
Abstract: The combination of a Legionella pneumophila culture isolation technique and macrophage infectivity potentiator (mip) gene-specific nested polymerase chain reaction (PCR) is pivotal for effective routine use in an environmental water system laboratory. Detection of Legionella organisms in 169 environmental samples was performed by using modified buffered charcoal yeast extract (MBCYE) agar for conventional culture. Nested PCR specific for L. pneumophila was performed using boiled genomic DNA extracts from filtered and Chelex 100-treated water samples, or by using silica-gel membrane spin column-eluted DNA from concentrated pond, canal and river samples. Overall, the nested PCR was twelvefold more sensitive than the culture method. The target amplicons (471 basepairs) of all 4 biochemically characterized L. pneumophila isolates were sequenced. They had homology at the DNA and protein levels to 3' proximity of the mip-coding gene of L. pneumophila deposited in genome databases. E |
Published in:Microbiol.Immunol. 51[8], 777-785. 2007. |
Last updated: September, 2010
Copyright © 2008 Faculty of Tropical Medicine, Mahidol University. All rights reserved. Webmaster : tmwww@mahidol.ac.th |
||