{"id":831,"date":"2025-10-20T10:11:09","date_gmt":"2025-10-20T03:11:09","guid":{"rendered":"http:\/\/10.11.101.25\/wordpress\/tmdr-lab\/?page_id=831"},"modified":"2025-10-20T16:02:26","modified_gmt":"2025-10-20T09:02:26","slug":"specimens-collection-en","status":"publish","type":"page","link":"http:\/\/10.11.101.25\/wordpress\/tmdr-lab\/specimens-collection-en\/","title":{"rendered":"Specimen Collection"},"content":{"rendered":"\t\t
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  • \n \n \n \n Blood and Serum Samples<\/span> <\/li>\n
  • \n \n \n \n Specimen Collection for COVID-19<\/span> <\/li>\n
  • \n \n \n \n Specimen Collection for Mpox virus<\/span> <\/li>\n <\/ul>\n <\/div>\n \n
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    Blood and Serum Samples<\/h4>\n
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    1. Labelling Blood Collection Tubes\n
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      1. Affix the patient label in a straight line on the tube; do not wrap it around in a spiral.<\/li>\n \t
      2. Ensure the tube color code and fill line remain visible after the label is applied.<\/li>\n \t
      3. If the sticker is longer than the tube, trim the excess\u2014keeping the HN and patient name visible\u2014or fold the extra portion back on itself.<\/li>\n<\/ol>\n<\/li>\n \t
      4. Blood Collection Guidelines\n
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        1. Verify that the patient name on the request form matches the label on the blood collection tube.<\/li>\n \t
        2. Ask the patient’s full name before every blood draw.<\/li>\n \t
        3. Avoid applying the tourniquet for more than 1 minute to prevent alteration of test results. If vacuum collection is not used, do not remove the cap\u2014instead, pierce through the cap with the needle and allow the vacuum to draw the blood without pushing the syringe plunger.<\/li>\n<\/ol>\n<\/li>\n \t
        4. Multiple Tube Collection: Order and Mixing Instructions\n
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          1. Clotted blood tube: Invert 5 times<\/li>\n \t
          2. EDTA tube: Invert 8 times<\/li>\n \t
          3. Heparin tube: Invert 8 times<\/li>\n \t
          4. Citrate tube: Invert 8 times<\/li>\n \t
          5. Plasma or serum must be separated by centrifugation immediately after blood collection.<\/li>\n<\/ol>\n<\/li>\n \t
          6. Eligible Submitters – Patients from the Hospital for Tropical Diseases, other hospitals, research institutions, and private companies may submit specimens in appropriate collection tubes according to the specimen type (see table below).<\/li>\n<\/ol>\nSpecimen Types, Volumes, and Storage Conditions\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
            No.<\/strong><\/th>\nTest<\/strong><\/th>\nSpecimen Type<\/strong><\/th>\nVolume<\/strong><\/th>\nStorage Conditions<\/strong><\/th>\n<\/tr>\n
            1<\/td>\nDetection and serotyping of Dengue virus using Real-time RT-PCR<\/td>\nEDTA blood, Clotted blood, EDTA plasma, Serum<\/td>\nAdults and children >12 years: 2 mL\nChildren <12 years: minimum 1 mL<\/td>\n2\u20138\u202f\u00b0C for up to 6 hours If delayed, separate serum or plasma and store at \u221220\u202f\u00b0C to \u221280\u202f\u00b0C<\/td>\n<\/tr>\n
            Collect at least 0.5\u20131 mL of plasma or serum in a microcentrifuge tube. Ensure the cap is tightly closed and sealed with parafilm.<\/td>\n<\/tr>\n
            2<\/td>\nDetection of anti-Dengue virus IgM and IgG antibodies using ELISA test<\/td>\nEDTA blood, Clotted blood, Citrate blood, Heparinized blood, Plasma, Serum<\/td>\n\u0e1cAdults and children >12 years: 2 mL\nChildren <12 years: minimum 1 mL<\/td>\n2\u20138\u202f\u00b0C for up to 24 hours If delayed, separate serum or plasma and store at \u221220\u202f\u00b0C to \u221280\u202f\u00b0C<\/td>\n<\/tr>\n
            Collect at least 0.5\u20131 mL of plasma or serum in a microcentrifuge tube. Ensure the cap is tightly closed and sealed with parafilm.<\/td>\n<\/tr>\n
            3<\/td>\nDetection of Chikungunya virus using Real-time RT-PCR<\/td>\nEDTA blood, otted blood, EDTA plasma, Serum<\/td>\nEDTA plasma: 2 ml, EDTA blood 5 ml, Clotted blood 5 ml, Serum 2 ml<\/td>\n2\u20138\u202f\u00b0C for up to 24 hours If delayed, separate serum or plasma and store at \u221220\u202f\u00b0C to \u221280\u202f\u00b0C<\/td>\n<\/tr>\n
            4<\/td>\nDetection of Zika virus using Real-time RT-PCR<\/td>\nUrine, EDTA blood, Clotted blood, EDTA plasma, Serum<\/td>\nUrine 2 ml, EDTA plasma 2 ml , Serum 2 ml, EDTA blood 5 ml, Clotted blood 5 ml<\/td>\n2\u20138\u202f\u00b0C for up to 24 hours If delayed, separate serum\/plasma\/urine and store at \u221220\u202f\u00b0C to \u221280\u202f\u00b0C<\/td>\n<\/tr>\n
            5<\/td>\nDiagnosis of Parasites Using the Stool Concentration Technique<\/td>\nStool sample<\/td>\nCollect about a thumb-sized amount of stool, sampling from different areas, especially those with color variations or abnormal characteristics.<\/td>\nStool samples collected at home should be submitted to the laboratory within 4 hours. If required, they can be stored in a regular refrigerator for up to 1 day.<\/td>\n<\/tr>\n
            6<\/td>\nDiagnosis of Parasites Using the Direct Wet Smear Technique<\/td>\nStool sample<\/td>\nCollect about a thumb-sized amount of stool, sampling from different areas, especially those with color variations or abnormal characteristics.<\/td>\nStool samples collected at home should be submitted to the laboratory within 4 hours. If required, they can be stored in a regular refrigerator for up to 1 day.<\/td>\n<\/tr>\n
            7<\/td>\nDetection of Pinworm Using the Scotch Tape Technique<\/td>\nPinworm eggs in the perianal region<\/td>\nCollect a perianal specimen by applying clear adhesive tape immediately after the patient wakes up, before bathing. Transfer the tape onto a microscope slide for examination<\/td>\nSlides can be stored at room temperature for no longer than 24 hours.<\/td>\n<\/tr>\n
            8<\/td>\nMalaria Diagnosis Using Thick and Thin Blood Films<\/td>\nEDTA Blood<\/td>\n– Collect 10\u202f\u00b5L of blood by finger-prick from the patient.\n– Collect 2\u20133\u202fmL of EDTA blood.<\/td>\nSpecimens can be stored at room temperature for no longer than 24 hours.<\/td>\n<\/tr>\n
            9<\/td>\nDetection of Occult Blood in Stool Using Immunochromatographic Test<\/td>\nStool sample<\/td>\n– Collect approximately a thumb-sized portion of stool, making sure to sample from throughout the stool mass. Select areas with different colors or any abnormal portions, such as those containing mucus or blood.\n– Use freshly passed stool, collected directly into the toilet or a clean container.\n– Avoid consuming the following foods and supplements 48\u201372 hours prior to stool collection: beetroot, carrots, bananas, grapes, cantaloupe, cucumber, broccoli, cauliflower, horseradish, turnip, various mushrooms, red meat, fish, chicken, iron supplements, pig\u2019s blood, chicken blood, and vitamin C or vitamin C-containing beverages.<\/td>\nStool samples collected at home should be submitted to the laboratory within 4 hours. If required, they can be stored in a regular refrigerator for up to 1 day.<\/td>\n<\/tr>\n
            10<\/td>\nDetection of Zika, Chikungunya, and Dengue viruses using Multiplex Real-time RT-PCR<\/td>\nEDTA blood, Clotted blood<\/td>\n\u0e1cAdults and children >12 years: 2 mL\nChildren <12 years: minimum 1 mL<\/td>\n2\u20138\u202f\u00b0C for up to 24 hours If delayed, separate serum or plasma and store at \u221220\u202f\u00b0C to \u221280\u202f\u00b0C<\/td>\n<\/tr>\n
            Collect at least 0.5\u20131 mL of plasma or serum in a microcentrifuge tube. Ensure the cap is tightly closed and sealed with parafilm.<\/td>\n<\/tr>\n<\/tbody>\n<\/table> <\/div>\n\t\t \n
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            Specimen Collection and Handling for COVID-19 Diagnosis (SARS-CoV-2 by Real-time RT-PCR)<\/h4>

            To ensure accuracy and reliability in laboratory testing, specimen collectors are advised to follow these guidelines:<\/p>

            1. Preparation Prior to Sample Collection: