EDUCATION

1992 - 1996

B.Sc., Biochemistry and Biochemical Technology, Chiang Mai University, Thailand

1997 – 2000

M.Sc., Immunology, Mahidol University, Thailand

2003 – 2008

Ph.D., Microbiology, Mahidol University, Thailand

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CHRONOLOGY OF EMPLOYMENT

2020-present

Associate Professor, Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University

2016-2020

Assistant Professor, Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University

2014-2016

Lecturer, Department of Clinical Medicine, Faculty of Tropical Medicine, Mahidol University

2009-2014

Research Fellow, Emerging Respiratory Viruses Section Laboratory of Infectious Diseases, NIAID, NIH, USA

• Competed NIH BSL-3 and BSL-4 training.
• Evaluation of replication, immunogenicity and protective efficacy of a live attenuated cold adapted pandemic H1N1 influenza virus vaccine in non-human primates.
• Study the role of plasmacytoid dendritic cells on pathogenesis of high pathogenic avian influenza H5N1 virus.
• Developing new live attenuated H5N1 vaccine.
• Developing immune based treatment for influenza virus infection.
• Determination of cytokine profiles in influenza virus infected nasal epithelium cell culture by Bio-plex multiplex system.
• Developing flow cytometry based single cell sorting to identify the niches of live attenuated influenza vaccine (LAIV) replication among various upper respiratory tract cell types including epithelial and hematopoietic cells

2004 - 2009

Research Fellow, U.S. Military HIV Research Program, Henry M. Jackson Foundation, MD, USA

• Developed a high-throughput flow cytometry-based Antibody-Dependent Enhancement assay
• Developed absolute quantitative PCR assay for dengue viral RNA copy number enumeration and detection of replicative intermediate forms of dengue viral genome
• Investigate ADE of dengue infection in cell line systems, primary human monocytes and dendritic cells
• Study the role of glycosylation sites in dengue envelope protein using dengue mutant viruses in DC-SIGN-bearing cells (transfected cell line systems and primary human dendritic cells)
• Investigate the role of FcyRs (FcyRI, FcyRII) in dengue virus infection
• Investigate the effect of FcyRII isoforms in Antibody Dependent Enhancement in dengue virus infection
• Study the influence of FcyRIIa polymorphism in Antibody Dependent Enhancement in dengue infection
• Study the effect of DC-SIGN inhibitor (dendrimer) on dengue infection
• Study cytokine production in response to dengue infection using cytokine bead assay
• Assist in grant proposal, progress report and manuscript preparation and review
2000-2003 Research Assistant, Microbiology Department, Siriraj Hospital, Mahidol University, Thailand
• Studied T cell responses in HIV infected patient using Chromium Release CTL assay
• Designed HIV recombinant vaccinia constructs to support CTL assays in HIV infected Thai Cohort
• Studied cross-clade CTL responses using ELISpot assay under Merck adenovirus based HIV vaccine Trial Project
• Coordinator for Merck adenovirus based HIV vaccine Trial Project (Thailand Site)
• Established PCR based method to detect H5N1 infection in clinical specimens for the first endemic in Thailand
• Conducted full genome sequencing of H5N1 genome, along with full characterization and analysis for genetic evaluation of circulating H5N1 virus in Thailand
• Constructed H5N1 genome segments in vaccinia virus for use as a tool to study T cell responses in H5N1 infected patients and close contact cases
• Assisted in grant proposal, progress report and manuscript preparation