Name: Pornpan Pumirat
Position: Associate Professor
Department: Microbiology and Immunology
Indrawattana N, Santajit S, Seng R, Rungruengkitkun A, Kong-Ngoen T, Tunyong W, Thavorasak T, Reamtong O, Sricharunrat T, Chantratita N, Pumirat P. Deciphering the diversity, structure, and function of cycle-inhibiting factor in Burkholderia pseudomallei neuroinfection. Sci Prog. 2025 Jul-Sep;108(3):368504251369011.
Khan T, Toufiq M, Yurieva M, Indrawattana N, Jittmittraphap A, Kosoltanapiwat N, Pumirat P, Sukphopetch P, Vanaporn M, Palucka K, Syed Ahamed Kabeer B, Rinchai D, Chaussabel D. Automating candidate gene prioritization with large language models: from naive scoring to literature-grounded validation. Bioinformatics. 2025 Oct 2;41(10):btaf541.
Thavorasak T, Santajit S, Tunyong W, Kong-Ngoen T, Reamtong O, Ampawong S, Saelim N, Srisai T, Aiumurai P, Pumirat P, Chaicumpa W, Indrawattana N. CRISPR/Cas9-targeted smpB mutation revealing roles in biofilm formation, motility, and antibiotic susceptibility in Acinetobacter baumannii. PLoS One. 2025 Aug 4;20(8):e0329638.
Pumirat P, Santajit S, Tunyong W, Kong-Ngoen T, Tandhavanant S, Lohitthai S, Rungruengkitkun A, Chantratita N, Ampawong S, Reamtong O, Indrawattana N. Impact of AbaI mutation on virulence, biofilm development, and antibiotic susceptibility in Acinetobacter baumannii. Sci Rep. 2024 Sep 14;14(1):21521.
Lerdsittikul V, Apiratwarrasakul S, Atithep T, Withatanung P, Indrawattana N, Pumirat P, Chaiwattanarungruengpaisan S, Thongdee M. Isolation and characterisation of a novel Silviavirus bacteriophage promising antimicrobial agent against methicillin-resistant Staphylococcus aureus infections. Sci Rep. 2024 Apr 22;14(1):9251.
Lohitthai S, Rungruengkitkun A, Jitprasutwit N, Kong-Ngoen T, Duangurai T, Tandhavanant S, Sukphopetch P, Chantratita N, Indrawattana N, Pumirat P. Type VI Secretion System Accessory Protein TagAB-5 Promotes Burkholderia pseudomallei Pathogenicity in Human Microglia. Biomedicines. 2023; 11(11):2927. https://doi.org/10.3390/biomedicines11112927
Jitprasutwit N, Rungruengkitkun A, Lohitthai S, Reamtong O, Indrawattana N, Sookrung N, Sricharunrat T, Sukphopetch P, Chatratita N, Pumirat P. In Vitro Roles of Burkholderia Intracellular Motility A (BimA) in Infection of Human Neuroblastoma Cell Line. Microbiol Spectr. 2023 Aug 17;11(4):e0132023. doi: 10.1128/spectrum.01320-23.
Santajit S, Bhoopong P, Kong-Ngoen T, Tunyong W, Horpet D, Paehoh-Ele W, Zahedeng T, Pumirat P, Sookrung N, Hinthong W, Indrawattana N. Phenotypic and Genotypic Investigation of Carbapenem-Resistant Acinetobacter baumannii in Maharaj Nakhon Si Thammarat Hospital, Thailand. Antibiotics (Basel). 2023 Mar 15;12(3):580. doi: 10.3390/antibiotics12030580.
Duangurai T, Rungruengkitkul A, Kong-Ngoen T, Tunyong W, Kosoltanapiwat N, Adisakwattana P, Vanaporn M, Indrawattana N, Pumirat P. Phylogenetic analysis and antibiotic resistance of Escherichia coli isolated from wild and domestic animals at an agricultural land interface area of Salaphra wildlife sanctuary, Thailand. Vet World. 2022 Dec;15(12):2800-2809. doi: 10.14202/vetworld.2022.2800-2809.
Rungruengkitkun A, Jitprasutwit N, Muangkaew W, Suttikornchai C, Tandhavanant S, Indrawattana N, Ampawong S, Sukphopetch P, Chantratita N, Pumirat P. Cycle-Inhibiting Factor Is Associated with Burkholderia pseudomallei Invasion in Human Neuronal Cells. Biology (Basel). 2022 Oct 1;11(10):1439. doi: 10.3390/biology11101439.
Pewlaoo S, Phanthong S, Kong-Ngoen T, Santajit S, Tunyong W, Buranasinsup S, Kaeoket K, Thavorasak T, Pumirat P, Sookrung N, Chaicumpa W, Indrawattana N. Development of a Rapid Reverse Transcription-Recombinase Polymerase Amplification Couple Nucleic Acid Lateral Flow Method for Detecting Porcine Epidemic Diarrhoea Virus. Biology (Basel). 2022 Jul 6;11(7):1018. doi: 10.3390/biology11071018.
Santajit S, Kong-Ngoen T, Tunyong W, Pumirat P, Ampawong S, Sookrung N, Indrawattana N. Occurrence, antimicrobial resistance, virulence, and biofilm formation capacity of Vibrio spp. and Aeromonas spp. isolated from raw seafood marketed in Bangkok, Thailand. Vet World. 2022 Jul;15(7):1887-1895. doi: 10.14202/vetworld.2022.1887-1895.
Kong-Ngoen T, Santajit S, Tunyong W, Pumirat P, Sookrung N, Chaicumpa W, Indrawattana N. Antimicrobial Resistance and Virulence of Non-Typhoidal Salmonella from Retail Foods Marketed in Bangkok, Thailand. Foods. 2022 Feb 24;11(5):661. doi: 10.3390/foods11050661.
Kaewpan A, Duangurai T, Rungruengkitkun A, Muangkaew W, Kanjanapruthipong T, Jitprasutwit N, Ampawong S, Sukphopetch P, Chantratita N, Pumirat P. Burkholderia pseudomallei pathogenesis in human skin fibroblasts: A Bsa type III secretion system is involved in the invasion, multinucleated giant cell formation, and cellular damage. PloS One. 2022;17 (2):e0261961.
eroviruses associated with acute flaccid paralysis in Thailand in 2013 and 2014. Virol J. 2021;18(1):153.
Tunyong W, Arsheewa W, Santajit S, Kong-Ngoen T, Pumirat P, Sookrung N, Chaicumpa W, Indrawattana N. Antibiotic Resistance Genes Among Carbapenem-resistant Enterobacterales (CRE) Isolates of Prapokklao Hospital, Chanthaburi Province, Thailand. Infect Drug Resist. 2021;14:3485-3494.
Santajit S, Kong-Ngoen T, Chongsa-Nguan M, Boonyuen U, Pumirat P, Sookrung N, Chaicumpa W, Indrawattana N. Human Single-Chain Antibodies That Neutralize Elastolytic Activity of Pseudomonas aeruginosa LasB. Pathogens. 2021;10(6):765.
Chamchoy K, Pumirat P, Reamtong O, Pakotiprapha D, Leartsakulpanich U, and Boonyuen U. Functional analysis of BPSS2242 reveals its detoxification role in Burkholderia pseudomallei under salt stress. Sci Rep. 2020; 10(1):10453.
Duangurai T, Reamtong O, Rungruengkitkun A, Srinon V, Boonyuen U, Limmathurotsakul D, Chantratita N, Pumirat P. In vitro passage alters virulence, immune activation and proteomic profiles of Burkholderia pseudomallei. Sci Rep. 2020; 10:8320.
Reamtong O, Indrawattana N, Rungruengkitkun A, Thiangtrongji T, Duangurai T, Chongsa-nguan M, and Pumirat P. Altered proteome of a Burkholderia pseudomallei mutant defective in short-chain dehydrogenase affects cell adhesion, biofilm formation and heat stress tolerance. PeerJ. 2020; 8:e8659.
Santajit S, Seesuay W, Mahasongkram K, Sookrung N, Pumirat P, Ampawong S, Reamtong O, Chaicumpa W, Indrawattana N. Human single-chain variable fragments rescue Pseudomonas aeruginosa C12-HSL quorum-sensing molecule triggered mitochondria-regulated apoptosis. Front Microbiol. 2020; 11: 1172.
Tinyou A, Chaimon S, Phuphisut O, Kobpornchai P, Malaithong P, Poodeepiyasawat A, Ieamsuwan I. Ruangsittichai J, Pumirat P, Dekumyoy P, Reamtong O, and Adisakwattana P. Molecular cloning and characterization of serine protease inhibitor from food-borne nematode, Gnathostoma spinigerum. Acta Trop. 2019 Dec 4:105288.
Chamchoy K, Pakotiprapha D, Pumirat P, Leartsakulpanich U and Usa Boonyuen. Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays. BMC Biochemistry. 2019 Apr 8;20(1):4. doi: 10.1186/s12858-019-0108-1.
Pumipuntu N, Tunyong N, Chantratita N, Diraphat P, Pumirat P, Sookrung N, Chaicumpa W and Indrawattana N. Novel Staphylococcus aureus complex isolated from subclinical bovine mastitis in Central and Northeast Thailand. PeerJ. 2019 Mar 14;7:e6587. doi: 10.7717/peerj.6587. eCollection 2019.
Sricharunrat T, Pumirat P, and Leaungwutiwong P. Mechanisms of Oral Tolerance. Asian Pac J Allergy Immunol. 2018 Dec;36(4):207-216. doi: 10.12932/AP0848.
Paksanont S, Sintiprungrat K, Yimtin T, Pumirat P, Peacock SJ, and Chantratita N. Effect of temperature on Burkholderia pseudomallei growth, proteomic changes, motility and resistance to stress environments. Sci Rep. 2018 Jun 15;8(1):9167.
Pinweha P, Pumirat P, Cuccui J, Jitprasutwit N, Muangsombut V, Boonyuen U, Thiennimitr P, Vattanaviboon P, Cia F, Willcox S, Bancroft GJ, Wren BW, Korbsrisate S. Nitrate acquisition under anaerobic condition by the BPSL1039-1040 ABC transporter contributes to biofilm formation, intracellular survival and attenuation in the murine model of Burkholderia pseudomallei infection. PLoS One. 2018 May 17;13(5):e0196202.
Duangurai T, Indrawattana N and Pumirat P. Burkholderia pseudomallei Adaptation for Survival in Stressful Conditions. Biomed Res Int. Volume 2018, Article ID 3039106.
Gatedee J, Muangman S, Pumirat P, Mahakunkijcharoen Y, Prasertsincharoen N, Kritsiriwuthinan K. Isolation and biological characterization of bacteriophages which infect Aeromonas hydrophila. Journal of Applied Animal Science Vol.10 No.3 September-December 2017
Pumirat P, Vanaporn M, Boonyuen U, Indrawattana N, Rungruengkitkun A, Chantratita N. Effects of sodium chloride on heat resistance, oxidative susceptibility, motility, biofilm and plaque formation of Burkholderia pseudomallei. Microbiologyopen. 2017 Aug;6(4).
Vanaporn M, Sarkar-Tyson M, Kovacs-Simon A, Ireland PM, Pumirat P, Korbsrisate S, et al. Trehalase plays a role in macrophage colonization and virulence of Burkholderia pseudomallei in insect and mammalian hosts. Virulence. 2017 Jan 2;8(1):30-40.
Pumirat P, Broek CV, Juntawieng N, Muangsombut V, Kiratisin P, Pattanapanyasat K, et al. Analysis of the prevalence, secretion and function of a cell cycle-inhibiting factor in the melioidosis pathogen Burkholderia pseudomallei. PLoS One. 2014;9(5):e96298.9
Pumirat P, Broek CV, Juntawieng N, Muangsombut V, Kiratisin P, Pattanapanyasat K, et al. Analysis of the prevalence, secretion and function of a cell cycle-inhibiting factor in the melioidosis pathogen Burkholderia pseudomallei. PLoS One. 2014;9(5):e96298.
Pumirat P, Cuccui J, Stabler RA, Stevens JM, Muangsombut V, Singsuksawat E, et al. Global transcriptional profiling of Burkholderia pseudomallei under salt stress reveals differential effects on the Bsa type III secretion system. BMC Microbiol. 2010 Jun 14;10:171.
Pumirat P, Saetun P, Sinchaikul S, Chen ST, Korbsrisate S, Thongboonkerd V. Altered secretome of Burkholderia pseudomallei induced by salt stress. Biochim Biophys Acta. 2009;1794:898-904. IF = 3.17
Muangsombut V, Suparak S, Pumirat P, Damnin S, Vattanaviboon P, Thongboonkerd V, et al. Inactivation of Burkholderia pseudomallei bsaQ results in decreased invasion efficiency and delayed escape of bacteria from endocytic vesicles. Arch Microbiol. 2008;190:623-31. IF = 1.975