Dr. Pannamthip Pitaksajjakul Lecturer

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pannamthip.pit@mahidol.ac.th
+66 (0) 2354 9100, Ext 2042

Background

Dr. Pannamthip Pitaksajjakul carries out both teaching and research; in both cases focusing on the production and characterization of monoclonal antibodies against Dengue virus. She teaches both M.Sc. and Ph.D. students; she currently has three research projects with two Master students, and three doctoral students.

Dr. Pannamthip Pitaksajjakul has a number of international collaborators including the Center for Biotechnology, Osaka University, Japan which focuses on recombinant IgG production in mammalian cells, and large scale production of antibody.

Her primary research focus has two major emphases. First, using genetic engineering to enhance the activity of therapeutic antibody and second, optimizing the production yield of recombinant IgG expressed in mammalian cell for further large scale production.

Affiliations

Department of Social and Environmental Medicine
Center of Excellence for Antibody Research (CEAR)

Research

Dr. Pannamthip Pitaksajjakul’s research is designed to meet the vision of Center of Excellence for Antibody Research (CEAR). One significant project involves producing promising candidates of therapeutic human monoclonal antibodies (HuMAbs) for dengue treatment. Using genetic engineering technology, she and her CEAR colleagues have created new modified HuMAbs which are capable of neutralizing all four serotypes of dengue virus while avoiding the infection enhancement (ADE) associated with exposure to multiple dengue serotypes. Related research has resulted in the production of optimized HuMAb-secreting mammalian cells which are stable and suitable for scaling up to larger production.

A grant from the Thailand Research Fund is financing work on a dengue test kit based on an immunochromatography test. This is part of a collaboration with Chiba University, Chiba, Japan. Based on this research, CEAR received a Research award in year 2015 and Inventor award in the year 2016, both from the National Research Council of Thailand.

Qualifications

2004 Ph.D. (Tropical Medicine) Tropical Medicine, Mahidol University
2001 M.Sc. (Tropical Medicine) Tropical Medicine, Mahidol University
1999 B.Sc. (Chemistry) Srinakarinwirot University

Research Areas

  • Antibody production and engineering
  • Molecular biology

Publications

A full list of publications by Dr. Pannamthip Pitaksajjakul can be found at NCBI.

Selected Publications

Molecular genetic characterization of rabies virus glycoprotein gene sequences from rabid dogs in Bangkok and neighboring provinces in Thailand, 2013-2014.
Benjathummarak S, Fa-ngoen C, Pipattanaboon C, Boonha K, Ramasoota P, Pitaksajjakul P
Arch Virol. 2016 May;161(5):1261-71.
DOI: 10.1007/s00705-016-2789-8. Epub 2016 Feb 18. PMID: 26887972

Expression of enhancing-activity-free neutralizing antibody against dengue type 1 virus in plasmid-inoculated mice.
Yamanaka A, Pitaksajjakul P, Ramasoota P, Konishi E
Vaccine. 2015 Nov 9;33(45):6070-7.
DOI: 10.1016/j.vaccine.2015.07.089. Epub 2015 Aug 7. PMID: 26259543

Effect of Temperature on Fimbrial Gene Expression and Adherence of Enteroaggregative Escherichia coli.
Hinthong W, Indrawattana N, Pitaksajjakul P, Pipattanaboon C, Kongngoen T, Tharnpoophasiam P, Worakhunpiset S.
Int J Environ Res Public Health. 2015 Jul 23;12(8):8631-43. D
DOI: 10.3390/ijerph120808631.PMID: 26213951

Chikungunya virus was isolated in Thailand, 2010.
Sasayama M, Benjathummarak S, Kawashita N, Rukmanee P, Sangmukdanun S, Masrinoul P, Pitaksajjakul P, Puiprom O, Wuthisen P, Kurosu T, Chaichana P, Maneekan P, Ikuta K, Ramasoota P, Okabayashi T, Singhasivanon P, Luplertlop N.
Virus Genes. 2014 Dec;49(3):485-9.
DOI: 10.1007/s11262-014-1105-5. Epub 2014 Aug 12. PMID: 25113745

Antibody germline characterization of cross-neutralizing human IgGs against 4 serotypes of dengue virus.
Pitaksajjakul P, Benjathummarak S, Pipattanaboon C, Wongwit W, Okabayashi T, Kuhara M, Misaki R, Fujiyama K, Ramasoota P
Biochem Biophys Res Commun. 2014 Apr 4;446(2):475-80.
DOI: 10.1016/j.bbrc.2014.02.131. Epub 2014 Mar 14. PMID: 24637211